Table 1. Primer sequences and their respective annealing temperatures for both p53 amplification and nucleotide sequencing.

Region for amplification	Primer annealing temperature (°C)	Forward primer sequence	Reverse primer sequence
Exons 5–8	59	CACTTGTGCCCTGACTTTCAAC	AAAAGTGAATCTGAGGCATAAC
Exon 5	60	CCGCGCCATGGCCATCT	GCGCTCATGGTGGGGG
Exon 6	65	GTCCCCAGGCCTCTGATTCCTC	TAACCCCTCCTCCCAGAGACCCCAG
Exon 7	60	CTTGCCACAGGTCTCCCCAA	AGGGGTCAGCGGCAAGCAGA
Outer exon 8	60	GGACAGGTAGGACCTGATTTCC	AAAAGTGAATCTGAGGCATAAC
Inner exon 8	60	ACTGCCTCTTGCTTCTCTTTTCCTATCC	CTTGGTCTCCTCCACCGCTTCTTG