Table 1. Real-time quantitative PCR primers and reaction conditions.

Clone		Primer			PCR
Name	Acc. no.	Name	Sequence	Location	Size (bp)	T (°C), [MgCl2] (mM), [NTP] (μM)
EMP1	NM_001423.1	EMP1-2F	GACCTCATGCCATGGTCTTT	1393–1412	237	62, 4, 0.5
		EMP1-2R	CTGCATTGAGGGGAATCCTA	1556–1575
PIGR	AF272149.2	TB6-1F	CCACCGTGGAGATCAAGATT	1532–1551	186	62, 4, 0.5
		TB6-1R	CAGCCCGTGTTATTCCACTT	1669–1688
PON2	NM_000305	AEY125-F	GCCAACAATGGGTCTGTTCT	993–1012	198	62, 4, 0.5
		AEY126-R	TGGGTCAATGTTGCTGGTTA	1171–1190
Apol2	AF305225	AEY131-F	AGGCAGATGAGCTCCGTAAA	363–382	185	62, 4, 1
		AEY131-R	GACCTGCTCAACTCCTCTG	528–547
DRG1	D87953	AEY139-F	GCTTTGGTCAGAGTGAATTGAA	2717–2736	182	62, 4, 0.5
		AEY140-R	CCGATCCCCGACTTTTCTAC	2879–2898
PSMD8	NM_004159	AEY147-F	GAAGGAAGATGGTTGGGTA	981–998	189	62, 4, 0.5
		AEY147-R	TCTCTTTGGCTCAGGCTAGG	1150–1169
RP1-68D18	BM285393	EAW253-F	TGCAAGTCACCACAACAGGT	153 589–153 608	185	62, 4, 0.5
		EAW254-R	AGCCTTGCATAAATGGCTGT	153 753–153 773
HSPC150	NM_014176	AEY151-F	TGTTCTCAAATTGCCACCAA	390–409	191	62, 4, 1
		AEY152-R	TTGCATGCTTCTCTGTCCAC	516–580
RPLP0	M17885	RPP0-3F	GAAGGCTGTGGTGCTGATGG	224–243	103	62, 3, 1.5
		RPLP0-R	CCGGATATGAGGCAGCAGTT	307–326

Listed above are the clone names and accession numbers; the primer name, sequence (5′–3′) and location on the sequence associated with the accession number; and the PCR product size and reaction conditions (annealing temperature and concentrations of MgCl₂ and NTP).