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Time course of displacement of Rh-CP by nonlabeled C-peptide. After incubation of cells with 5 nM Rh-CP for 60 min, 5 μM nonlabeled C-peptide was added, and FCS measurements were carried out at given time intervals. Each data point represents the mean of at least six measurements. Log scale for the binding displacement process is shown as Inset.
