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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Exp Neurol. 2007 Nov 1;210(1):14–29. doi: 10.1016/j.expneurol.2007.10.011

Figure 2. ADAS cell transplants attenuated loss of nigral and striatal tyrosine hydroxylase immunoreactivity and rotational behavior in 6-OHDA lesioned rats.

Figure 2

Comparison of midbrain dopaminergic (tyrosine hydroxylase-positive) cell bodies and fiber immunoreactivity at 4 weeks post-lesion in control (unlesioned, n = 4), 6-OHDA-lesioned (n = 4), 6-OHDA-lesioned with sham (n = 4) transplant, 6-OHDA-lesioned with passage 2 naïve ADAS cells transplant (n = 5), or passage 2 differentiated ADAS cells transplant (n = 5). A, Representative rostral midbrain sections from 6-OHDA/Sham and 6-OHDA/naïve ADAS transplanted rats were stained with an antibody against tyrosine hydroxylase (TH). The 6-OHDA-lesioned hemisphere in the sham-transplanted rat displayed significant loss of TH-immunoreactivity in SNpc relative to the contralateral (unlesioned) side whereas 6-OHDA-lesioned hemisphere of rat that also received a naïve ADAS cell transplant displayed significant sparing of TH-positive cell bodies and fibers. Scale bar = 400 um. B, Stereological estimates of nigral DA neuron number, and C, Relative density of striatal TH-positive fibers confirm the neuroprotective effects of the autologous grafts of naïve and differentiated ADAS cells against 6-OHDA-induced loss of nigral TH-positive dopaminergic neurons (see Materials and Methods). Values expressed for neuron number and striatal density are group means ± S.D. Values were compared by one-way ANOVA followed by Tukey-Kramer HSD post-hoc test. Groups with different letters are significantly different at p<0.05. D, Rotational behavior was monitored for 20 minutes after amphetamine administration at 2.5 mg/kg i.p. (see Materials and Methods) as an indirect measure of striatal dopamine depletion induced by 6-OHDA. 6-OHDA-lesioned rats that received passage 2 naïve ADAS cells (black diamonds, n = 5) or NDM-treated ADAS cells (black triangles, n = 5) displayed attenuated amphetamine-induced rotational behavior compared to 6-OHDA-lesioned sham-transplanted (black squares, n = 4) rats. Values expressed are the group means ± S.D. Values were compared by one-way ANOVA followed by Tukey-Kramer HSD post-hoc test. Groups marked with an asterisk (*) are significantly different from sham at p < 0.05.