Table 1.
Summary of Immunocytochemical analyses of cell marker expression in naive (undifferentiated) and differentiated ADAS cells
| Markers | D10 Medium | D10→ EGF/FGF-2 2d | D10→ EGF/FGF-2→ NDM 4d | D10→ EGF/FGF-2→ N2/VPA 4d | NSA Prolif→ NSA Differentiation 4d |
|---|---|---|---|---|---|
| Fibronectin | +++ | +++ | ++ | ++ | ++ |
| Vimentin | + | ++ | ++ | + | ++ |
| Nestin* | + | ++ | + | + | + |
| VE Cadherin* | − | + | ++ | ++ | + |
| Neuro D* | − | +/− | ++ | +++ | + |
| Tuj1* | − | +/− | ++ | ++ | ++ |
| NSE* | − | − | ++ | ++ | ++ |
| NeuN | − | − | +/− | +/− | ++ |
| MAP2b | − | − | +/− | +/− | ++ |
| GABA | − | − | + | +/− | +/− |
| ChAT* | − | − | +/− | +/− | +/− |
| TH* | − | − | +/− | +/− | +/− |
| GFAP* | − | + | +/− | +/− | + |
| S100β* | − | +/− | +/− | +/− | +/− |
| O4 | − | +/− | +/− | +/− | +/− |
| SMA* | ++ | ++ | +/− | +/− | ++ |
| TrkB | − | − | + | + | + |
| OX-42 | − | +/− | − | − | − |
| p75NTR | − | +/− | − | − | +/− |
| MBP | − | − | − | − | − |
*
denotes that mRNA confirmed by real time QPCR. Studies were performed on ADAS cells at passages 2 through 4.
+++ = robust expression in >50% of the cells
++ = strong expression in ~ 50% of the cells
+ = detectable expression in < 50% of the cells
+/− = detectable expression in small percentage (<10%) of the cells
− = no detectable expression