DomA induces p38 phosphorylation in mouse CGNs. A. CGNs from Gclm (+/+) and Gclm (−/−) mice were incubated with 0.1 uM DomA for 1 h, and p38 activation was determined at the indicated times by Western analysis using an antibody recognizing phosphorylated p38. The anti beta-actin antibody was used to confirm equal protein loading in each gel lane, while an anti-p38 antibody was used to determine levels of p38 protein. B. Quantitation of the experiments shown in panel A, representing the mean (+/− SD) of three independent experiments from three separate cultures. *Significantly different from control, p < 0.05; **Significantly different from control, p<0.01.