Figure 3.
DomA decreases the phosphorylation of Erk 1/2 (p42/p44) in mouse CGNs. A. CGNs from Gclm (+/+) and Gclm (−/−) mice were incubated with 0.1 uM DomA for 1 h, and Erk 1/2 activation was determined at the indicated times by Western analysis using an antibody recognizing phosphorylated Erk 1/2. An anti-Erk 1/2 antibody was used to determine levels of Erk 1/2 proteins. B. Quantitation of the experiments shown in panel A, representing the mean (+/− SD) of three independent experiments from three separate cultures. *Significantly different from control, p < 0.05; **Significantly different from control, p<0.01.