Figure 3.

Detection of known villus epithelial and Paneth cell biomarkers. Relative transcriptional levels of previously characterized Paneth cell and villus epithelial biomarkers. Fold differences in expression between the crypt and villus epithelial regions are shown for HD5, HD6, trypsin, lysozyme, PLA2IIA (crypt epithelium) and carboxyesterase-2, PEP carboxykinase, aminopeptidase N, apolipoprotein A-IV, aldolase-B (villus epithelium) to demonstrate the magnitude of differential expression between the 2 cellular compartments and provide supportive evidence that the appropriate cells were captured by LCMD. Statistical validation of the differences in expression levels were determined by student t-test (P value ≤ 0.05).