Figure 4.

Localization of epithelial biomarkers by immunohistochemistry. Expression of HD5 in crypt epithelium and aldolase-B in villus epithelium is shown by immunohistochemistry analysis. HD5 (A) and aldolase-B (B) protein expression were evaluated by immunohistochemistry using peroxidase detection of mouse monoclonal anti-HD5 and anti-aldolase-B antibodies in consecutive serial tissue sections. Expression of HD5 was detected exclusively in Paneth cells, while aldolase-B was observed primarily in the epithelial cells of the villus region, providing supportive protein level evidence for the transcriptional differences elucidated by microarray and RT-PCR based assays.