Figure 5.
Copy number and tissue distribution of Ad-mFVIII genomes as determined by semiquantitative PCR. (Upper) Autoradiographs of PCR products after amplification of genomic DNA with Ad-mFVIII-specific primers are shown. The tissues analyzed are indicated above each lane. The predicted PCR product is 597 bp in length. Negative control samples include DNA from uninjected carrier females (XHX) and DNA from an adult mouse injected i.v. with AdCa18Luc. As a positive control for Ad-mFVIII PCR, hepatic DNA from an adult hemophilic animal injected i.v. with Ad-mFVIII was amplified and is shown in lane 17. (Lower) PCR amplification with mouse β-actin-specific primers of the same DNA samples shown above. The dose of Ad-mFVIII previously delivered i.p. in each group of samples appears at the bottom of the figure.