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. Author manuscript; available in PMC: 2008 Aug 24.
Published in final edited form as: J Biol Chem. 2007 Jun 12;282(34):24743–24751. doi: 10.1074/jbc.M700663200

Figure 5. Dependence of glucose-dependent dissociation V-ATPase complexes containing mutant forms Vph1p or Stv1p on intracellular environment.

Figure 5

Yeast strains MM112, MM112 vps21Δ and MM112 vps27Δ expressing either the R735K or R735Q mutant forms of HA-Vph1p (top panels) or the R795Q or R795K mutant forms of HA-Stv1p (bottom panels) were tested for glucose-dependent dissociation as described in the legend to Fig. 2. The results of a representative experiment are shown on the left (panel A for Vph1p and panel C for Stv1p) and the average of three independent determinations are shown on the right (panel B for Vph1p and panel D for Stv1p). Results are again expressed as the ratio of assembly observed in the absence of glucose divided by the assembly observed in the presence of glucose, with error bars corresponding to average deviations.