Figure 7.

Bax-deficient neutrophils activate the Bax gene under conditions of cytokine withdrawal. (A) Freshly purified Bax-deficient inflammatory diseases neutrophils (0; cystic fibrosis, see Fig. 2A) were cultured without cytokine support for the indicated times, and Bax protein expression was determined by flow cytometry. (B) Freshly purified normal neutrophils (0) were cultured in the presence of G-CSF for 30 h to down-regulate intracellular Bax protein levels (see Fig. 3A). Data are representative of five independent experiments.