Table 2.
Exp. | Conditions | Neutrophil death, % |
---|---|---|
1 | No treatment | 47 |
GM-CSF | 61 | |
Supernatant of patient 1 | 63 | |
Supernatant of patient 1 + anti-G-CSF/anti-GM-CSF | 41 | |
Anti-G-CSF/anti-GM-CSF | 46 | |
2 | No treatment | 50 |
GM-CSF | 71 | |
Supernatant of patient 4 | 60 | |
Supernatant of patient 4 + anti-G-CSF/anti-GM-CSF | 53 | |
Anti-G-CSF/anti-GM-CSF | 47 | |
3 | No treatment | 53 |
GM-CSF | 67 | |
Supernatant of patient 5 | 66 | |
Supernatant of patient 5 + anti-G-CSF/anti-GM-CSF | 55 | |
Anti-G-CSF/anti-GM-CSF | 49 |
Control neutrophils were cultured at the indicated conditions for 36 h before neutrophil death measurements. The patient numbers correspond to those in Table 1. The concentration of the neutralizing mAbs was 5 μg/ml each.