Table 2.
Proteins identified through proteomic analysis of A. thaliana mature stems
| Predicted or known functionsa | Accession | Spot numberb |
|---|---|---|
| Proteins acting on polysaccharides | ||
| GH family 1 | At2g44450 | 7 |
| GH family 1 | At3g18080 | 13, 14, 15 |
| GH family 1 | At5g54570 | 47 |
| GH family 1 (thioglucoside hydrolase 1) (TGG1) | At5g26000 | 41 |
| GH family 3 (BXL1) (beta-xylosidase 1) | At5g49360 | 5, 9, 11, 13 |
| GH family 3 (BXL2) (beta-xylosidase 2) | At1g02640 | 13, 15 |
| GH family 3 | At5g20950 | 5, 6, 8 |
| GH family 16 (xyloglucan endotransferase) (EXGT-A1) (At-XTH4) | At2g06850 | 13,14, 15 |
| GH family 16 (xyloglucan endotransferase) (Meri5) (At-XTH24) | At4g30270 | 15 |
| GH family 17 | At3g07320 | 6, 7 |
| GH family 17 | At5g56590 | 11, 13 |
| GH family 17 | At4g31140 | 33 |
| GH family 19 (chitinase), carbohydrate-binding module family 18 (chitin binding function) | At2g43570 | 31 |
| GH family 20 (N-acetyl-beta-glucosaminidase) | At1g65590 | 7, 10 |
| GH family 20 (N-acetyl-beta-glucosaminidase) | At3g55260 | 22 |
| GH family 28 (polygalacturonase) | At1g80170 | 8, 9, 10 |
| GH family 28 (polygalacturonase) | At3g07830 | 24 |
| GH family 28 (polygalacturonase) | At3g16850 | 29 |
| GH family 31 (alpha-glucosidase) (AtGLU1) | At5g11720 | 9, 13 |
| GH family 31 (alpha-xylosidase) (XYL1) | At1g68560 | 17 |
| GH family 32 (beta-fructofuranosidase) (ATBETAFRUCT4) | At1g12240 | 28 |
| GH family 35 (beta-galactosidase) (BGAL1) | At3g13750 | 8 |
| GH family 35 (beta-galactosidase) (BGAL9) | At2g32810 | 10 |
| GH family 35 (beta-galactosidase) (BGAL10) | At5g63810 | 7, 13 |
| GH family 38 (alpha-mannosidase) | At5g13980 | 13 |
| GH family 38 (alpha-mannosidase) | At3g26720 | 42 |
| GH family 51 (alpha-L-arabinofuranosidase) | At3g10740 | 27 |
| GH family 79 (endo-beta-glucuronidase/heparanase) | At5g07830 | 10 |
| GH family 79 (endo-beta-glucuronidase/heparanase ) | At5g34940 | 7 |
| CE family 8 (pectin methylesterase) | At3g43270 | 10 |
| CE family 13 (pectin acylesterase) | At5g23870 | 9 |
| Oxido-reductases | ||
| homolog to SKU5 (SKS4) (multicopper oxidase domain) | At4g22010 | 6 |
| homolog to SKU5 (SKS5) (multicopper oxidase domain) | At1g76160 | 7, 8 |
| homolog to SKU5 (SKS6) (multicopper oxidase domain) | At1g41830 | 7, 8, 12 |
| homolog to SKU5 (SKS7) (multicopper oxidase domain) | At1g21860 | 7, 12, 13 |
| homolog to SKU5 (SKS17) (multicopper oxidase domain) | At5g66920 | 10 |
| SKU5 (multicopper oxidase domain) | At4g12420 | 3, 6, 7, 8, 9 |
| homolog to peroxidase (AtPrx22) | At2g38380 | 11 |
| homolog to peroxidase (AtPrx28) | At3g03670 | 49 |
| homolog to peroxidase (AtPrx33) | At3g49110 | 11, 13 |
| homolog to peroxidase (AtPrx34) | At3g49120 | 23 |
| homolog to C. pepo ascorbate oxidase (P37064) | At5g21100 | 11 |
| homolog to berberine bridge enzyme (S)-reticulin:oxygen oxido-reductase | At4g20830 | 13 |
| homolog to germin (AtGER3) | At5g20630 | 32 |
| Proteins with interacting domains | ||
| homolog to carrot EDGP and tomato XEGIP | At1g03230 | 9, 10 |
| homolog to carrot EDGP and tomato XEGIP | At1g03220 | 39 |
| expressed protein (LRR repeats) | At1g33590 | 26 |
| expressed protein (LRR repeats) | At1g33600 | 8, 9 |
| expressed protein (LRR repeats) | At2g42800 | 14 |
| polygalacturonase inhibiting protein (PGIP2) | At5g06870 | 13, 15 |
| homolog to lectin (legume lectin beta domain) | At1g53070 | 13 |
| homolog to lectin (legume lectin beta domain) | At1g53080 | 13, 14, 15 |
| homolog to lectin (legume lectin beta domain) | At3g16530 | 1, 13, 14, 15 |
| homolog to lectin (curculin-like) | At1g78850 | 7, 8 |
| homolog to lectin (curculin-like) | At1g78860 | 7, 8 |
| homolog to lectin (curculin-like) | At5g18470 | 7, 8, 10 |
| Signalling | ||
| fasciclin-like arabinogalactan protein (AtFLA8) | At2g45470 | 1, 13, 14, 15 |
| fasciclin-like arabinogalactan protein (AtFLA13) | At5g44130 | 30 |
| Proteases | ||
| homolog to aspartyl protease | At1g09750 | 11, 12, 13 |
| homolog to aspartyl protease | At3g52500 | 8, 9 |
| homolog to aspartyl protease | At5g07030 | 10, 11, 12, 13 |
| homolog to aspartyl protease | At3g54400 | 21 |
| homolog to serine protease | At1g30600 | 7 |
| homolog to serine protease | At2g39850 | 50 |
| homolog to serine protease | At4g21630 | 12, 13, 14, 15 |
| homolog to serine protease | At4g21650 | 10, 13, 14, 15 |
| homolog to serine protease | At5g67360 | 16 |
| homolog to serine protease | At3g14067 | 18 |
| xylem serine peptidase 1 (XSP1) | At4g00230 | 9 |
| homolog to serine carboxypeptidase (SCPL11) | At2g22970 | 14, 15 |
| homolog to serine carboxypeptidase (SCPL12) | At2g22920 | 15 |
| homolog to serine carboxypeptidase (SCPL34) | At5g23210 | 12, 13 |
| Miscellaneous | ||
| homolog to anther specific proline-rich protein APG (lipase acylhydrolase domain) | At1g54000 | 13 |
| homolog to anther specific proline-rich protein APG (lipase acylhydrolase domain) | At1g67830 | 12 |
| homolog to purple acid phosphatase | At2g16430 | 7 |
| homolog to purple acid phosphatase | At2g27190 | 13 |
| homolog to purple acid phosphatase | At5g34850 | 19 |
| homolog to Chlamydomonas reinhardtii apospory associated protein (aldose-1-epimerase domain) | At4g25900 | 13 |
| homolog to glycerophosphoryl diester phosphodiesterase | At4g26690 | 35 |
| Unknown function | ||
| expressed protein | At1g21680 | 5, 6 |
| expressed protein | At1g21670 | 2, 3 |
| expressed protein | At3g14920 | 14, 15 |
| expressed protein (DUF642) | At4g32460 | 44, 13 |
| expressed protein (DUF642) | At5g11420 | 10, 11, 12 |
| expressed protein (DUF642) | At5g25460 | 12 |
| expressed protein (DUF1005) | At4g29310 | 13 |
| expressed protein (DUF1184) | At4g18080 | 46 |
| expressed protein (DUF1680) | At5g12950 | 5 |
| expressed protein (cupin domains) | At3g22640 | 34 |
| Intracellular proteins | ||
| homolog to thioredoxin | At1g21750 | 45 |
| GH family 1 | At2g25630 | 7 |
| homolog to serine protease | At2g05920 | 3, 9 |
| homolog to serine carboxypeptidase | At4g36195 | 43 |
| homolog to phosphorylase | At4g24350 | 12 |
| homolog to gamma-glutamyltranspeptidase | At4g39640 | 12 |
| homolog to aldo-keto reductase | At2g27680 | 37 |
| homolog to peroxiredoxin | At3g52960 | 25 |
| homolog to copper amine oxidase | At4g12990 | 21 |
| expressed protein (PH domain) | At2g30880 | 36 |
| expressed protein (RNA recognition motif) | At3g52980 | 40 |
| expressed protein | At2g41950 | 48 |
| expressed protein (CBS domains) | At3g48530 | 38 |
a
Functions are predicted as described in Experimental
b
Spot number refers to Fig. 1.