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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: J Neuroimmunol. 2008 Mar 6;195(1-2):47–59. doi: 10.1016/j.jneuroim.2008.01.006

Figure 2.

Figure 2

Characterization of primary astrocyte cultures derived from the olfactory bulb of young adult and reproductive senescent female rats. Primary astrocytes cultures were immunoreactive for GFAP (red: a,b) and were counter-stained with the nuclear dye, Hoechst (blue). Primary astrocyte cultures derived from young adult (c) and reproductive senescent females (d) were not immunoreactive for the neuronal marker, microtubule-associated protein-2 (MAP2). A small proportion (5-8%) of cells in these primary cultures derived from young adults (e,g) and reproductive senescent females (f,h) were immunopositive for the microglial markers, ionized-calcium binding adapter molecule 1 (Iba1) and the cell surface glycoprotein Mac-1 (CD11b). Iba-1 and CD11b immunopositive cells and their associated nuclei stained with Hoechst are indicated by white arrows. Immunohistochemistry with the nuclear dye Hoechst is shown in the bottom panel (c-h). Bar: 50 μm.