Table 1.
5% | 10% | 17% | |||||||
P value | FP | TP | P value | FP | TP | P value | FP | TP | |
Raw PM and MM | 0.008 | 1,098 | 19,398 | 0.025 | 2,291 | 20,182 | 0.060 | 4,300 | 20,687 |
GC-RMA PM and MM | 0.024 | 1,127 | 20,013 | 0.111 | 2,372 | 20,607 | 0.216 | 4,136 | 20,944 |
GC-RMA MM threshold | 0.037 | 1,107 | 19,969 | 0.111 | 2,392 | 20,621 | 0.235 | 4,202 | 21,003 |
The number of false positives (FPs) and true positives (TPs) is for a given percentage of false positives out of the total number of positives (5%, 10% and 17% but percentages for each method are not exact due to P value granularity) and the corresponding P value cutoff for the MAS 5.0 present/absent calls. The calculations are based on a total of 60,624 true negatives (10,104 empty probesets in 6 replicates) and 23,436 true positives (3,906 probesets that can be aligned to transcripts in six replicates). The default P value cutoff is 0.060 for separating marginally present from absent transcripts.