Figure 2.

(A) Surface expression of GP Ib-IX on GP V wt, +/− and −/− platelets. WP were incubated with Ab #3584 (1:1,000 dilution) and analyzed by flow cytometry. The data are representative of six animals of each genotype. Control IgG is on the left. (B) Western analysis of GP Ib-IX expression on GP V wt, +/− and −/− platelets. Lysed WP (5 × 10⁷) were electrophoresed, transferred, and incubated with Ab#3584 (1:1,000) followed by ECL. Four individual mice of each genotype are shown (C) Binding of GP V wt, +/− and −/− platelets to immobilized human vWf. Pooled WP were incubated as described in Methods. The data shown are the average of duplicates and are representative of three such experiments. (D) Binding of FITC–vWf to GP V wt, +/− and −/− platelets. Pooled PRP was incubated with FITC–vWf and botrocetin and analyzed by flow cytometry. The data are representative of three experiments done in duplicate.