Figure 4.

(A) Thrombin-induced aggregation in GP V wt +/− and −/− platelets. WP were prepared from six to ten mice of each genotype, which were littermate controls. Platelet aggregation was determined in a lumi-aggregometer. Five experiments were carried out, and four worked in the manner shown. (B) FACS analysis of surface αΙΙbβ3 levels in GP V wt, +/− and −/− platelets. PRP was incubated with Ab #41 (10 μg/ml), and samples were analyzed by flow cytometry. The data are representative of six individual mice per genotype. Control IgG is on the left. (C) Western analysis of total αΙΙbβ3 levels in GP V wt, +/− and −/− platelets. WP lysates were electrophoresed, transferred to nitrocellulose, and incubated with Ab #41 (10 μg/ml) followed by ECL. Four individual mice of each genotype are shown.