FIG. 2.

Dilution curve for anti-rABA MAb binding to the rABA. The wells of 96-well ELISA plates were coated with 1 μg of rABA, and the protein was incubated with 100-μl aliquots of various dilutions of the hybridoma culture supernatants for 3 h at room temperature. The extent of binding of the antibodies to the immobilized antigens was determined by the addition of rabbit α-mIg-HRP, followed by the addition of tetramethylbenzidine-H₂O₂ and the measurement of the absorbance at 450 nm.