FIG. 4.

Western blotting analysis. Two micrograms of each of the proteins (rABA, abrin, and A. precatorius agglutinin I [APA-I]) was electrophoresed on a 12.5% sodium dodecyl sulfate gel under reducing conditions and then transferred electrophoretically onto a nitrocellulose membrane. After the plates were blocked with 0.2% BSA, the blots were probed with A-chain-specific MAbs, followed by rabbit α-mIg-HRP, and subsequently visualized with the substrate diaminobenzidine-H₂O₂. Lane M, molecular size markers.