TABLE 2.
IFN-γ ELISPOT assay results at 8 weeks after initial vaccination
| Groupa | Mean change in no. of IFN-γ SFCs/106 PBMCsb |
|---|---|
| Nonvaccinated | 35 ± 13 |
| BCG | 131 ± 37 |
| BCG-CFP-DDA-PIM2 | 205 ± 51c |
| BCG-CFP-DDA-MPL | 212 ± 39c |
| BCG-CFP-DDA-Pam3CSK4 | 213 ± 94c |
| BCG-DDA-MPL | 222 ± 48c |
a
Four animals per group were randomly selected for the IFN-γ ELISPOT assay.
b
The change in the numbers of SFCs represents the number of SFCs for the bovine PPD-stimulated PBMCs minus the number of SFCs for the medium control PBMCs/106 cells. The mean number of SFCs for the medium control PBMCs/106 cells was 81 ± 19.
c
The results are significantly different from the results for the nonvaccinated group (P < 0.05).