FIG. 3.

Plasma 7S antibody reactivity to Ni-Sepharose affinity-enriched recombinant CFPHV glycoprotein H among 10 “known positive” green turtles over time. “Known positives” were experimentally infected turtles that developed FP at inoculation sites. CFPHV infection was confirmed by PCR of tumor DNA. (A) ELISA scores plotted against time relative to the start of the experiment. (B) ELISA scores plotted against time relative to the first detection of FP in each animal (time zero). Time points sampled prior to time zero were from turtles that did not have tumors, while those sampled after time zero were from turtles with detectable tumors. Reference ELISA scores (means ± standard deviations) for 10 “known negative” controls (▪) and the positive assay control sample (□) are included for comparison. The cutoff value (0.12) corresponds to 3 standard deviations above the mean score of “known negative” pool sample.