FIG. 4.

Immunoblot demonstrating the anti-gH antibody-binding specificity of ELISA-positive turtle plasma. The antigen used was recombinant baculovirus-infected insect cell culture lysate coexpressing glycoprotein H and glycoprotein L (lanes 1 to 3) and control baculovirus culture lysate (lane 4). Plasma samples were diluted 1:20 in blocking buffer. Lane 1, blocking buffer-only control; lane 2, ELISA negative control; lanes 3 and 4, ELISA-positive field sample. Turtle 7S antibodies were detected with monoclonal antibody HL858 (1 μg ml⁻¹) followed by peroxidase-conjugated goat anti-mouse IgG (diluted 1:5,000) in blocking buffer. The ELISA-positive sample specifically reacted with proteins consistent with gH (∼75 kDa) and gL (∼20 kDa).