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. Author manuscript; available in PMC: 2008 Nov 2.
Published in final edited form as: Cell. 2008 May 2;133(3):510–522. doi: 10.1016/j.cell.2008.02.046

Figure 6. Nkx6.1 promotes Slit1 expression in astrocytes and overrides its repression by Pax6.

Figure 6

Sections through the spinal cord of E12 chick embryos electroporated with the constructs indicated above the diagram, and labeled with the antibodies (AMV, NF1A) or cRNA probes (cSlit1, cReelin) indicated to the left. Abbreviations as in Fig 4. (M–R) and (EE-JJ) are the same panels as in (G–L) and (Y-DD), respectively, but include NFIA expression. (S–X) and (KK-PP) are higher power images of the boxed areas indicated in (M–R) and (EE-JJ), respectively. Arrows in (S–X) and (KK-PP) indicate double positive cells, arrowheads NFIA single positive cells. (QQ) The percentage of Slit1+ NFIA+ cells is significantly increased on the electroporated (E) side of Nkx6.1 embryos, relative to either the control (C ) side of the same embryos (***, p=.0003), or to the E side of GFP embryos (***, p<.001). (n=4 embryos per condition, two independent experiments). (RR) Bars represent mean ± S.E.M. from the E side only. The data from embryos electroporated with Pax6 alone (blue bars) or Nkx6.1 alone (orange bars) are reproduced from Figs. 4CC and 6QQ, respectively, and included for comparative purposes. The percentage of Slit1+NFIA+ cells in embryos mis-expressing both Nkx6.1 and Pax6 is significantly higher than in GFP controls (Slit1, ***, p<.001), and is similar to that in embryos expressing Nkx6.1 alone (QQ). (n=4 embryos per condition, 2 independent experiments).