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Oligonucleotides used in this study
| Oligonucleotide | Sequence |
|---|---|
| KanA | CGCACGTCAAGACTGTCAAG |
| KanB | TCGTATGTGAATGCTGGTCG |
| 5′trp1::kanlox | GCCAAGAGGGAGGGCATTGGTGACTATTGAGCACGTGAGTATAC |
| 3′trp1::kanlox | GAACTATTTTTATATGCTTTTACAAGACTTGAAATTTTCCTTGC |
| 5′TRP1 | TGGCATGTCTGGCGATGATA |
| 3′TRP1 | CGCCTGTGAACATTCTCTTC |
| XbaI-5′MDH3 | GCTCTAGAAACATGGTCAAAGTCGCAA |
| 3′MDH3-SalI | AGCTGTCGACTCAAGAGTCTAGGATGAAACTCT |
| XbaI-5′SpMAE1 | GCTCTAGACATGGGTGAACTCAAGGA |
| 3′SpMAE1-SalI | ACGCGTCGACTTAAACGCTTTCATGTTCA |
