. 2008 Mar 7;74(9):2873–2881. doi: 10.1128/AEM.02080-07

TABLE 2.

Steady-state kinetic parameters for low-molecular-weight compounds of MnP2 variants^a

MnP2 wild type or variant	Avg K_m (μM) ± SD			Avg V_max (μmol/min/mg) ± SD
MnP2 wild type or variant	Mn²⁺	H₂O₂	VA	Mn²⁺	H₂O₂	VA
Wild type	22.3 ± 2.8	10.3 ± 0.2	5,080 ± 222	43.1 ± 2.6	50.8 ± 5.9	4.4 ± 0.1
W170A	21.0 ± 2.9	9.9 ± 0.8	ND	96.9 ± 0.3	45.6 ± 4.1	ND
R263N	22.3 ± 2.2	9.8 ± 1.0	5,059 ± 274	98.1 ± 8.4	41.1 ± 1.0	14.1 ± 0.7
Q266F	25.9 ± 1.4	9.3 ± 0.6	2,322 ± 221	89.4 ± 4.4	43.8 ± 4.8	2.5 ± 0.5
V166/168L	26.6 ± 4.7	7.0 ± 0.4	4,361 ± 339	74.8 ± 6.7	31.1 ± 3.3	3.6 ± 0.4

The kinetic parameters of the wild-type and recombinant MnP2 enzymes for Mn²⁺, H₂O₂, and VA were determined as described previously (16, 34, 38). ND, not detected. The averages were determined from three independent experiments.