TABLE 2.

Characteristics of RCA cluster sequences found in nonbloom temperate environments

% Similarity to LE17	Source	Clone name	GenBank accession no.	No. of base pairs	Referencea
100	Saline lake, Japan	1w	AB154432	524	28
100	Japan coast	HB02-8b	AB265989	169	22
100	Coast of Georgia	GAI-36	AF007259	400	18
100	Long Island Sound	pC2-12	AF055225	298	16
100	Coastal North Sea	OTU_E	AF207853	534	65
99.0	Coastal Georgia	EC-I1	AF287022	392	11
100	Weser estuary, Germany	WM11-37b	AF497861	523	54
100	English Channel	DGGE band 5	AJ242822	515	15
99.0	Mediterranean coast, France	S1-090-F-C-Nr1	AJ508432	523	Unpublished
99.5	North Sea, Germany	UNHYB_26b	AJ630678	450	53
100	Oregon coast	HTCC152c	AY102029	647	10
99.7	Marine sediment	s29	AY171302	1,018	27
100	Coast of Skagerrak Sea	SKA55c	AY317122	429	57
99.3	Black Sea oxycline	BSBd6-20/40mb	AY360519	829	64
98.8	Estuary, Portugal	RAN-63	AY499446	592	23
99.1	North Sea, United Kingdom	PEL-52	AY550815	550	14
100	Estuary, Massachusetts	PI_RT343b	AY580461	816	1
100	Coastal salt pond, United States	SP_C23	AY589480	783	56
99.8	Estuary, Oregon	LS-F4	AY628657	608	4
100	Wadden Sea, Germany	Flo-37b	AY684343	465	20
100	Salt marsh, Georgia	SIMO-662b	AY712199	451	Unpublished
100	Coast of Scotland	FFW402	AY828410	719	Unpublished
99.5	Fjord, Norway	LUR12	AY960287	570	48
99.7	California coast	SPOTSAPR01_5m124b	DQ009296	1,400	6
99.9	Eutrophic bay, Washington	PB1.27b	DQ071074	1,386	29
99.7	Northeast Pacific, 2,500-m depth	CTD005-37B-02	DQ513055	1,427	24
99.2	Coast of Chile	Chili1-G4b	DQ669616	404	43
99.6	O2 minimum, Chilean coast	ESP10-K27II-52	DQ810325	1,301	Unpublished
99.6	North Sea, Denmark	NS5	DQ839250	539	Unpublished
99.9	Coast of China	PV2-27	EF215774	918	Unpublished
100	North Atlantic, 1,700 m	001733_3285_1268b	NAe	60	59
99.5	Coast, North Carolina	OM65b	U70682	643	46
99.6	Northwest Mediterranean	T41_191	DQ436604	751	Unpublished
100	Plum Island Sound, United States	PIdgge30	AY308694	132	12
99.6	Coastal Mediterranean	Isolate a	EF018061	498	Unpublished
100	Gulf of Mained	1097156605048	NA	694	50
99.8	Brown's Bank, Mained	1097156701473	NA	1,226	50
99.9	Bedford Basin, Nova Scotiad	1097159073554	NA	935	50
100	Newport Harbor, RId	1097169034943b	NA	1,470	50
98.7	Cape May, NJd	1097173026724	NA	946	50
100	NAgs Head, NCd	109720503350	NA	1,213	50

^a“Unpublished” indicates that sequences found in GenBank are not yet associated with a publication.

^bRepresentative sequence among several RCA cluster clones.

^cReported dilution-to-extinction culture but unable to successfully propagate.

^dSample from the GOS metagenomic analysis.

^eNA, not available.