FIG. 5.
PCR analysis of the F. tularensis subsp. holarctica (LVS) iglC1::ltrBLl iglC2::ltrBLl mutant. Using combinations of gene-specific primers (primers a and b), an intron-specific primer (primer c), and FPI-I- and FPI-II-specific primers (primers d and e) (A), PCR analysis was performed on genomic DNA from F. tularensis subspecies holarctica strains LVS (wild type [WT]) (lanes 2, 4, 6, 8, and 10) and KKL1 (lanes 3, 5, 7, 9, and 11) (B). Primers (Table 2): a, iglCNcoIF; b, iglCNdeIR; c, iglC427 428a-EBS2; d, FTL_0105R; e, FTL_1152R.