TABLE 3.

Phylogenetic assignment of bacterial 16S rRNA gene sequences generated from sediment downstream of the WWTP outfall^a

Phylum	Class	Order or family	No. of sequences foundb
			Upstream of 400 m	Downstream of 400 m
Proteobacteria	Alphaproteobacteria	Caulobacteraceae	2
		Sphingomonadaceae	2
		Unclassified	1	3
	Betaproteobacteria	Burkholderiales	9	2
		Hydrogenophilales		2
		Rhodocyclales	6	2
		Unclassified	10	2
	Gammaproteobacteria	Oceanospirillales		2
		Methylococcaceae		3
		Unclassified		2
	Deltaproteobacteria	Desulfobulbaceae	1
		Cystobacterineae		3
Nitrospirae	Nitrospirales	Nitrospiraceae	2
Bacteroidetes	Sphingobacteria	Unclassified		2
Actinobacteria		Actinobacteridae	2
Gemmatimonadetes		Gemmatimonadetes	2
Verrucomicrobia	Verrucomicrobiales	Verrucomicrobiaceae	1	2

^aSediment from above and below sampling position 5 (see Fig. 1), 400 m downstream of the WWTP outfall, was sampled.

^b16S rRNA gene sequences were matched to the family or class of bacteria according to the classification of the Ribosomal Database Project.