TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or characteristics | Source or reference |
|---|---|---|
| Plasmids | ||
| pVO155 | Derivative plasmid of pUC19 containing the promoterless gus (uidA) gene; Kmr Ampr | 36 |
| pRK600 | Cmr, traRK2, oriColE1 | 16 |
| pIJ3225 | hrp cluster of X. campestris pv. campestris cloned in pLAFR1 | 3 |
| Wild-type strains | ||
| CFBP4834-R | X. fuscans subsp. fuscans CFBP4834-R wild-type strain; yellow strain producing the typical fuscous pigment; Rifr | 22 |
| ATCC 33913 | X. campestris pv. campestris ATCC 33913 wild-type strain; Rifr | 11 |
| 85.10 | X. axonopodis pv. vesicatoria wild-type strain; Rifr | 6 |
| 306 | X. citri subsp. citri wild-type strain | 11 |
| K-12 | Escherichia coli wild-type strain | 12 |
| DH5α | E. coli F φ980dlacZ:M15 recA1 endA1 gyrA96 thi-1 hsdR17 (rK− mK+) supE44 relA1 deoR (lacZYA-argF)U169 | 19 |
| C600 | E. coli C600 wild-type strain; Rifr | 34 |
| Mutants | ||
| 4834HRCJ | CFBP4834-R hrcJ(420)a::pVO155; Rifr Kmr | This study |
| 4834HRCR | CFBP4834-R hrcR(320)::pVO155; Rifr Kmr | This study |
| 4834HRCT | CFBP4834-R hrcT(799)::pVO155; Rifr Kmr | This study |
| 4834HRCV | CFBP4834-R hrcV(1501)::pVO155; Rifr Kmr | This study |
| 4834HRPB2 | CFBP4834-R hrpB2(298)::pVO155; Rifr Kmr | This study |
| 4834HRPG | CFBP4834-R hrpG(625)::pVO155; Rifr Kmr | This study |
| 4834HRPX | CFBP4834-R hrpX(459)::pVO155; Rifr Kmr | This study |
| 33913HRCU | ATCC 33913 hrcU::pVO155; Rifr Kmr | Gift from M. Arlat |
| 33913HRPX | ATCC 33913 hrpX::pVO155; Rifr Kmr | Gift from M. Arlat |
a
Numbers in parentheses indicate the position from the start codon of pVO155 insertion in the target gene in X. citri subsp. citri 306.