TABLE 2.

PCR primers used in this study to construct and validate mutations in hrp genes of X. fuscans subsp. fuscans CFBP4834-R

Target gene	PCR primer		Relevant characteristic	PCR fragment size (bp)
	Namea	Nucleotide sequenceb
hrcJ	HrcJ-F	GGACTAGTCCTGCTGCATGCGGGCGTGGA	SpeI site	335
	HrcJ-R	CCGCTCGAGCGGGAAAGCGGGTCGTTGTTGG	XhoI site
	HrcJ-ext-F	CATGACGCTCATTCCTCCTGT	To be used with ProR	891
hrcR	HrcR-F	GGACTAGTCCGCTGGTGGTCATCATGCTGG	SpeI site	293
	HrcR-R2	CCGCTCGAGCGGTGTTTGAGGAGGAATTGC	XhoI site
	HrcR-ext-R	TCACCGATAGCTCAGAACCAGG	To be used with ProF	756
hrcT	HrcT-F	GGACTAGTCCCCTCGCAGGGCGTGTCGCTG	SpeI site	329
	HrcT-R2	CCGCTCGAGCGGGTCATCTGCACATTGTTGTA	XhoI site
	HrcT-ext-R	CGTTGGCGGCATCGTGCAAT	To be used with ProF	850
hrcV	HrcV-F	CGGGATCCCGATTCGCAAAAACGCCCCTGATT	BamHI site	454
	HrcV-R	GGTCTAGAGCCAGCAGACGCCGCAACACAT	XbaI site
	HrcV-ext-F	TGGCCAACGAGAACGAGACAAG	To be used with ProR	789
hrpB2	HrpB2-F	GGACTAGTCCATGACGCTCATTCCTCCTGT	SpeI site	357
	HrpB2-R	CCGCTCGAGCGGGCATCAACTTGATCTGCT	XhoI site
	HrcB2-ext-R	GGAAAGCGGGTCGTTGTTGG	To be used with ProF	896
hrpG	HrpG-F	GGACTAGTCCTGCGAGCTGCTGATCTTCGAT	SpeI site	467
	HrpG-R	CCGCTCGAGCGGTTGTAGATGTGCTGCTCCATGGT	XhoI site
	HrpG-ext-F	CACCAACCAGCATCCTGCTC	To be used with ProR	1,680
hrpX	HrpX-F	GGACTAGTCCGCCTACAGCTACATGATCACCAA	SpeI site	206
	HrpX-R2	CCGCTCGAGCGGCTTCGGCCAGCAGTTCGT	XhoI site
	HrpX-ext-R	TTACCGCTGCAAGGTCTCCATCGG	To be used with ProF	446
	ProR	TTCACGGGTTGGGGTTTCTACA	pVO155 primer
	ProF	GAGATCCCCAGCCCGCCTAATG	pVO155 primer

^aFor each target gene, the two first primers indicated were designed to construct the mutation. The third primer, which needs to be used with ProF or ProR, was designed for the validation of the mutation and for the specific identification of mutated strains.

^bUnderlined sequences indicate the relevant restriction site.