Skip to main content
. 2008 Mar 7;74(9):2627–2636. doi: 10.1128/AEM.02711-07

TABLE 1.

Quantitative PCR TaqMan primer and probe sequences

Gene Function Sequence
dps1 Sense primer 5′CGAGTCGCCGACATACTTCA3′
Antisense primer 5′GCGTCAGATGCAGATCGTTGT3′
Probea 5′(6FAM)AAGCGGCTCAGCGC(NFQ)3′
dps2 Sense primer 5′TGAGCCTGATCGCCAAACA3′
Antisense primer 5′GTGGACCGCCAGGAAGTG3′
Probea 5′(6FAM)CTGGAATGTCATCGGAC(NFQ)3′
sigF1 Sense primer 5′TGCTGATGTTGCGGTTCTTC3′
Antisense primer 5′ACGCGCTGGGCGATT3′
Probea 5′(6FAM)ATCGATGACCCAGACGG(NFQ)3′
sigF2 Sense primer 5′TTGGCCGGACGAGTATCAG3′
Antisense primer 5′CGAGGGCTGCCATCTGTT3′
Probea 5′(6FAM)TCACAGCACTCTTC (NFQ)3′
sigF3 Sense primer 5′AATCACTTCCCGACCTCGAA3′
Antisense primer 5′GGGTCATGTTGCCGAAGAA3′
Probeb 5′(6FAM)CACCGTGCTGGTCC (TAMRA)3′
ectA Sense primer 5′CGAGATACGGCGATCAACAA3′
Antisense primer 5′TGTCCATCGGGAAAATCGTT3′
Probea 5′(6FAM)CAAGAACTGTTCTCCC(NFQ)3′
DNApol IV Sense primer 5′GACAACAAGTTACGAGCCAAGATC3′
Antisense primer 5′CCTCCGTCAGCCGGTAGAT3′
Probea 5′(VIC)CGACGGACTTCGGCA (NFQ)3′
a

5′ 6FAM (6-carboxyfluorescein) or VIC fluorophore and 3′ nonfluorescent quencher.

b

5′ 6FAM (6-carboxyfluorescein) fluorophore and 3′ TAMRA (6-carboxytetramethylrhodamine) quencher.