TABLE 1.
Gene | Function | Sequence |
---|---|---|
dps1 | Sense primer | 5′CGAGTCGCCGACATACTTCA3′ |
Antisense primer | 5′GCGTCAGATGCAGATCGTTGT3′ | |
Probea | 5′(6FAM)AAGCGGCTCAGCGC(NFQ)3′ | |
dps2 | Sense primer | 5′TGAGCCTGATCGCCAAACA3′ |
Antisense primer | 5′GTGGACCGCCAGGAAGTG3′ | |
Probea | 5′(6FAM)CTGGAATGTCATCGGAC(NFQ)3′ | |
sigF1 | Sense primer | 5′TGCTGATGTTGCGGTTCTTC3′ |
Antisense primer | 5′ACGCGCTGGGCGATT3′ | |
Probea | 5′(6FAM)ATCGATGACCCAGACGG(NFQ)3′ | |
sigF2 | Sense primer | 5′TTGGCCGGACGAGTATCAG3′ |
Antisense primer | 5′CGAGGGCTGCCATCTGTT3′ | |
Probea | 5′(6FAM)TCACAGCACTCTTC (NFQ)3′ | |
sigF3 | Sense primer | 5′AATCACTTCCCGACCTCGAA3′ |
Antisense primer | 5′GGGTCATGTTGCCGAAGAA3′ | |
Probeb | 5′(6FAM)CACCGTGCTGGTCC (TAMRA)3′ | |
ectA | Sense primer | 5′CGAGATACGGCGATCAACAA3′ |
Antisense primer | 5′TGTCCATCGGGAAAATCGTT3′ | |
Probea | 5′(6FAM)CAAGAACTGTTCTCCC(NFQ)3′ | |
DNApol IV | Sense primer | 5′GACAACAAGTTACGAGCCAAGATC3′ |
Antisense primer | 5′CCTCCGTCAGCCGGTAGAT3′ | |
Probea | 5′(VIC)CGACGGACTTCGGCA (NFQ)3′ |
5′ 6FAM (6-carboxyfluorescein) or VIC fluorophore and 3′ nonfluorescent quencher.
5′ 6FAM (6-carboxyfluorescein) fluorophore and 3′ TAMRA (6-carboxytetramethylrhodamine) quencher.