TABLE 2.
Primer and probe sequences used in this studya
| Function or primer/probe | Sequence | Target gene (accession no.) | Reference(s) |
|---|---|---|---|
| Quantification of target mRNAs and genes concn | |||
| tceA_For | 5′-ATCCAGATTATGACCCTGGTGAA | tceA gene (AF228507) | 11, 13 |
| tceA_Rev | 5′-GCGGCATATATTAGGGCATCTT | tceA gene (AF228507) | 11, 13 |
| tceA_Probe | 5′-FAM-TGGGCTATGGCGACCGCAGG-TAMRA | tceA gene (AF228507) | 11, 13 |
| vcrA_For | 5′-CTCGGCTACCGAACGGATT | vcrA gene (AY322364) | 11 |
| vcrA_Rev | 5′-GGGCAGGAGGATTGACACAT | vcrA gene (AY322364) | 11 |
| vcrA_Probe | 5′-FAM-CGCACTGGTTATGGCAACCACTC-TAMRA | vcrA gene (AY322364) | 11 |
| bvcA_For | 5′- GGTGCCGCGACTTCAGTT | bvcA gene (AY563562) | 11 |
| bvcA_Rev | 5′-TCGGCACTAGCAGCAGAAATT | bvcA gene (AY563562) | 11 |
| bvcA_Probe | 5′-FAM-TGCCGAATTTTCACGACTTGGATGAAG-TAMRA | bvcA gene (AY563562) | 11 |
| Dhc16S_For | 5′-GGTAATACGTAGGGAAGCAAGCG | Dehalococcoides sp. 16S rRNA gene (CP000027) | 11 |
| Dhc16S_Rev | 5′-CCGGTTAAGCCGGGAAATT | Dehalococcoides sp. 16S rRNA gene (CP000027) | 11 |
| Dhc16S_Probe | 5′-VIC-ACATCCAACTTGAAAGACCACCTACGCTCACT-TAMRA | Dehalococcoides sp. 16S rRNA gene (CP000027) | 11 |
| FtL-RD1638I_For | 5′-CTGGGCCGAAACCAAAGA | Field sample FtL-RDase-1638 gene (bp 381 to 449)b | This study |
| FtL-RD1638I_Rev | 5′-CCCGTCCACTTCGGTTCAC | Field sample FtL-RDase-1638 gene (bp 381 to 449)b | This study |
| FtL-RD1638I_Probe | 5′-FAM-CCCGCTCCGGTAAAAACCCCTGA-TAMRA | Field sample FtL-RDase-1638 gene (bp 381 to 449)b | This study |
| FtL-RD1638II_For | 5′-TTCCTGTCAGAAGTGTGCAGATTC | Field sample FtL-RDase-1638 gene (bp 1056 to 1129)b | This study |
| FtL-RD1638II_Rev | 5′-GCAAATCCCATGAAGGTTCTTT | Field sample FtL-RDase-1638 gene (bp 1056 to 1129)b | This study |
| FtL-RD1638II_Probe | 5′-FAM-TGTCCGCCCCAGTGTATATCTAAAG-TAMRA | Field sample FtL-RDase-1638 gene (bp 1056 to 1129)b | This study |
| RNA standard synthesis | |||
| Dhc16S_T7_For | 5′-TAATACGACTCACTATAGGGGGTAATACGTAGGGAAGCAAGCG | Dehalococcoides 16S rRNA gene (CP000027) | This study |
| Unv_1492_Rev | 5′-GG(C/T)TACCTTGTTACGACTT | Dehalococcoides 16S rRNA gene (CP000027) | 4 |
| bvcA_T7_For | 5′-TAATACGACTCACTATAGGGGGTGCCGCGACTTCAGTT | bvcA gene (AY563562) | This study |
| bvcA_1270_Rev | 5′-ATTGTGGAGGACCTACCT | bvcA gene (AY563562) | 15 |
a
For, forward primer; Rev, reverse primer; underlined region, RNA polymerase T7 promoter sequence.
b
Nucleotide position according to the sequenced clone.