TABLE 1.
Experimental design for comparison of soil DNA extraction and purification methods
| Method | Soil pretreatment | DNA extraction | DNA purification | Reference or source |
|---|---|---|---|---|
| M | Mo Bio PowerSoil | Included in the kit | ||
| MV | Incubation with suspended CaCO3 | Mo Bio PowerSoil | Included in the kit | This work |
| S | Modified method of Miller et al. (17, 27) | 17 | ||
| SA | AlNH4(SO4)2, 50 mM in extraction buffer | Modified method of Miller et al. (17, 27) | 9 | |
| SC | CaCl2, 50 mM in extraction buffer | Modified method of Miller et al. (17, 27) | 4 | |
| SV | Incubation with suspended CaCO3 | Modified method of Miller et al. (17, 27) | This work | |
| SK | Modified method of Miller et al. (17, 27) | Incubation with CaCl2, GeneClean Turbo DNA kit | This work |