TABLE 1.
Toxicity of Cry1Aa α4 mutants toward M. sexta larvae
| Toxin | Toxin dose of 2 μg/ml
|
Toxin dose of 25 μg/ml
|
||
|---|---|---|---|---|
| % Mortalitya | Wt gain (mg)b | % Mortality | Wt gain (mg) | |
| Cry1Aa (wild type) | 95 ± 2 (54) | —c | — | — |
| L126C | 21 ± 4 (6)** | 12 ± 2 (6)** | 100 (6) | — |
| R127C | 99.3 ± 0.7 (6) | — | — | — |
| M130C | 99.3 ± 0.7 (6) | — | — | — |
| R131C | 0 (6)** | 59 ± 6 (6)* | 40 ± 9 (12)** | 11 ± 2 (12)** |
| I132C | 69 ± 6 (6)** | — | — | — |
| Q133C | 16 ± 6 (6)** | 16 ± 9 (6)** | 100 (6) | — |
| F134C | 71 ± 7 (6)** | — | — | — |
| N135C | 1.4 ± 0.9 (6)** | 65 ± 9 (6) | 22 ± 7 (6)** | 50 ± 10 (6)** |
| M137C | 96 ± 2 (6) | — | — | — |
| N138C | 19 ± 2 (6)** | 16 ± 3 (6)** | 100 (6) | — |
| S139C | 85 ± 5 (6) | — | — | — |
| A140C | 11 ± 5 (6)** | 32 ± 7 (6)** | 84 ± 6 (6)* | — |
| L141C | 93 ± 4 (6) | — | — | — |
| T142C | 11 ± 4 (6)** | 11 ± 2 (6)** | 98.6 ± 0.9 (6) | — |
| A144C | 0 (6)** | 149 ± 6 (6) | 6 ± 4 (6)** | 14 ± 2 (6)** |
| I145C | 95 ± 3 (6) | — | — | — |
| P146C | 0 (6)** | 44 ± 8 (6)** | 100 (6) | — |
| L147C | 0 (6)** | 180 ± 10 (6) | 0 (6)** | 50 ± 10 (6)* |
| A149C | 97 ± 2 (6) | — | — | — |
a
Values, corrected by Abbott's formula, are means ± standard errors of the means with the number of independent experiments shown in parentheses. Student t test differences shown were calculated relative to the values obtained for Cry1Aa (*, P < 0.05; **, P < 0.01).
b
Measured when mortality was lower than 50%. Student t test differences shown were calculated relative to the control values measured in the absence of toxin (*, P < 0.05; **, P < 0.01).
c
—, not tested.