TABLE 2.

Kinetic parameters of pore formation by Cry1Aa mutant toxins

Toxin	Delay (min)a,b		Rate (% vol recovery/min)b,c
	pH 7.5	pH 10.5	pH 7.5	pH 10.5
Cry1Aa (wild type)	0.48 ± 0.06	0.65 ± 0.02	30 ± 7	21 ± 1
L126C	>6	>6	1.11 ± 0.07*	1.8 ± 0.6**
R127C	0.38 ± 0.08	0.6 ± 0.1	26 ± 1	19 ± 2
M130C	0.35 ± 0.08	0.59 ± 0.07	25 ± 3	23 ± 1
R131C	>6	NAd	4.2 ± 0.9*	NA
I132C	1.0 ± 0.2	NA	5 ± 2*	NA
Q133C	2.2 ± 0.6*	>6	2.5 ± 0.2*	0.2 ± 0.3**
F134C	0.5 ± 0.1	0.79 ± 0.04*	16 ± 1	9 ± 5
M137C	0.49 ± 0.06	0.78 ± 0.08	20 ± 3	16 ± 4
N138C	>6	>6	0.9 ± 0.2*	0.8 ± 0.8**
L141C	0.42 ± 0.06	1.0 ± 0.1*	20 ± 3	6 ± 3**
I145C	1.1 ± 0.1**	3.8 ± 0.5**	4 ± 2*	1.3 ± 0.6**
P146C	>6	NA	0.9 ± 0.4*	NA
A149C	0.59 ± 0.06	1.17 ± 0.07**	15.2 ± 0.6	11 ± 2*

^aDelay is defined as the time required to reach 5% volume recovery. >6, mutants for which 5% volume recovery was not reached within the time course of the experiments.

^bSignificantly different from the corresponding value for Cry1Aa (*, P < 0.05; **, P < 0.01).

^cOsmotic swelling rates correspond to the maximal slopes of the curves.

^dNA, not active.