FIG. 6.

Intracellular adenine nucleotide levels during the incubation of a buffered and starved suspension of K. lactis CBS2359 with glucose at 30°C. Portions of the suspension were preequilibrated for 10 min at 30°C, after which glucose was added (at 0 min) to 27 mM. Intracellular adenine nucleotides (AXP; ATP, ADP, AMP) and total adenine nucleotides (total AXP = ATP + ADP + AMP) were measured and the EC was calculated after incubation with glucose for the times indicated and (plotted at −2.5 min) in samples taken from the yeast culture immediately before harvesting. For the incubation with glucose, the error bars indicate the ranges between duplicate assays. For the culture, the error bars correspond to the ranges between two samples. EC data (EC II) from a replicate experiment using independently grown yeast are also shown.