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. 2008 Apr 4;74(10):3216–3228. doi: 10.1128/AEM.02631-07

FIG. 7.

FIG. 7.

Construction of two shuttle vectors. (A) pLS203, E. coli pEM vector containing the 4-kb replication region of pSF118-20; (B) pLS208, E. coli pEM vector containing the 7-kb putative mobilization locus and replication region of pSF118-20.