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. 2008 Apr 4;74(10):3216–3228. doi: 10.1128/AEM.02631-07

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FIG. 8. — Characterization of pLS208 transconjugants. (A) Plasmid profiles of two representative transconjugants obtained from mating between L. plantarum NCIMB8826(pLS208) and either L. fermentum DSM20055 (pNZ8048) (T1) or L. salivarius JCM1045(pNZ8048) (T2). Lane M, DNA size markers; lane 1, undigested samples; lane 2, PstI-restricted plasmids. (B) Absence of pWCFS103 in representative L. fermentum transconjugant. PCR was performed with primers based on pWCFS103 (FF071, FF072B). Lane 1, template was total genomic DNA of L. fermentum transconjugant; lane 2, template was total genomic DNA of L. plantarum NCIMB8826; lane 3, negative control, distilled water; lane M, Hyperladder I (Bioline).