. 2008 Apr 4;74(10):3216–3228. doi: 10.1128/AEM.02631-07

TABLE 1.

Bacterial strains and plasmids

Strain or plasmid	Relevant properties^a	Source or reference
Strains
E. coli
Top10	F⁻mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL(Str^r) endA1 nupG	Invitrogen
L. lactis
MG1363	Plasmid-free derivative of L. lactis subsp. cremoris NCDO712	16
L. salivarius
UCC118	Ileocecal isolate from a human adult	12
LS201	pSF118-20-free derivative of strain UCC118	This work
L. plantarum
NCIMB8826	Isolated from human saliva, it is identical to the sequenced strain L. plantarum WCFS1, which is a single-colony isolate of strain NCIMB8826	NCIMB
L. fermentum
DSM20055	Isolated from saliva	DSM
Plasmids
pNZ8048	Cm^r, NcoI site has been used for translational fusions, nisin-induced gene expression vector	34
pVE6007	Cm^r, temperature sensitive, derivative of pWV01, lactococcal cloning vector	42
pCI341	Cm^r, replication probe vector	25
pLS201	Cm^r, pNZ8048 containing gene pemI and its own promoter region amplified from pSF118-20	This work
pEM	Em^r, pBluescript II SK(−) derivative in which amp was replaced by erm from pE194	Unpublished results^b
pLS202	Cm^r, pCI341 containing LSL_1963-LSL_1968	14
pLS203	Em^r, pEM containing LSL_1963-LSL_1967	This work
pLS204	Cm^r, pCI341 containing LSL_1965-LSL_1967	14
pLS205	Cm^r, pCI341 containing LSL_1965-LSL_1966	14
pLS206	Cm^r, pCI341 containing LSL_1965 and its 613-bp downstream region	14
pLS207	Cm^r, pCI341 containing LSL_1965	14
pLS208	Em^r, pEM containing LSL_1960-LSL_1967	This work
pLS209	Em^r; a derivative of pLS203 produced by PCR with primers FF122 and FF125, which has ClaI, NcoI, and HindIII sites in multiple cloning sites of pLS203	This work
pLS210	Em^r, a derivative of pLS203 for expressing luxABCDE under the promoter of cysK (LSL_1718)	This work
pLS211	Em^r, a derivative of pLS209 for expressing pemI and pemK (LSL_1984 and LSL_1985, respectively) under the native promoter of these loci	This work
pLS212	Cm^r, a derivative of pVE6007 for expressing pemI and pemK (LSL_1984 and LSL_1985, respectively) under the native promoter of these loci	This work
pLS213	Em^r, a promoter probe vector derived from pLS203 containing gfp⁺ from pZEP08	This work
pLS214	Em^r, a derivative of pLS213 for the production of GFP under the promoter of cysK (LSL_1718)	This work
pFT1	Amp^r, pUC19 containing the luxABCDE operon	Unpublished results^c
pZEP08	Cm^r, Km^r, a pBR322 derivative containing gfp⁺	23

Str^r, streptomycin resistant; Cm^r, chloramphenicol resistant; Em^r, erythromycin resistant; Amp^r, ampicillin resistant; Km^r, kanamycin resistant.

Contributed by Mary O'Connell Motherway, UCC.

Contributed by Ian Monk, UCC.