TABLE 4.
Segregational stabilities of constructs containing variously sized fragments of the replication region of pSF118-20 in lactic acid bacteria in the absence of antibiotic selection
Strain | Generation | % Stability at optimal growth temp for constructa:
|
|||||
---|---|---|---|---|---|---|---|
pLS202 | pLS203 | pLS204 | pLS205 | pLS206 | pLS207 | ||
L. lactis MG1363 | 50 | 46 | 3 | 33 | NR | NR | NR |
100 | 28 | 1 | 9 | NR | NR | NR | |
L. plantarum NCIMB8826 | 50 | 100 | 100 | 81 | 72 | 70 | 64 |
100 | 100 | 98 | 62 | 57 | 53 | 49 | |
L. salivarius LS201 | 50 | NT | 86 | NT | NT | NT | NT |
100 | NT | 81 | NT | NT | NT | NT | |
L. fermentum DSM20055 | 50 | NT | 94 | NT | NT | NT | NT |
100 | NT | 72 | NT | NT | NT | NT |
NR, no replication; NT, not tested.