TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant features | Reference or source |
|---|---|---|
| Z. mobilis ATCC 31821 | Wild-type strain | Laboratory stock |
| E. coli strains | ||
| FA114 | FA113 ΔtyrA ΔpheA | 5 |
| JM101 | supE thi Δ(lac-proAB) F′ | 6 |
| PB12 | JM101 Δ(ptsHI crr) Glc+ | 19 |
| PB12G | PB12 transformed with pJLBaroGfbr. | This study |
| PB12A | PB12 transformed with pJLBaroGfbr and pTrctyrA | This study |
| PB12C | PB12 transformed with pJLBaroGfbr.and pTrctyrC | This study |
| PB12CP | PB12 transformed with pJLBaroGfbr.and pTrctyrCpheACM | This study |
| Plasmids | ||
| pTrc99A | Cloning vector, carries bla and lacIq genes and trc promoter | 1 |
| pCR-BluntII-TOPO | Cloning vector, carries kan gene | Invitrogen (Carlsbad, CA) |
| pTOPOtyrA | tyrA gene cloned in pCR-BluntII-TOPO | This study |
| pTrctyrA | tyrA gene under control of the trc promoter | This study |
| pTOPOtyrC | tyrC gene cloned in pCR-Blunt II-TOPO | This study |
| pTrctyrC | tyrC gene under control of the trc promoter | This study |
| pTrcpheAfbra | pheAfbr gene codes for CM and prephenate dehydratase domains of P protein under control of the trc promoter | 3 |
| pTrctyrCpheACM | tyrC and pheACM under control of the trc promoter; pheACM codes for the CM domain of P-protein | This study |
| pTrctyrCpheACMINV | Same as pTrctyrCpheACM but pheACM is not in the correct orientation for its expression | This study |
| pJLBaroGfbr | aroGfbr under control of the lacUV5 promoter; carries lacIq and tet genes | 3 |
a
The superscript fbr indicates feedback inhibition resistance.