TABLE 2.
Genetic transfer of B. thailandensis chromosomal insertions
| Donor DNAa | Recipient strain | Transformation efficiency (no. of colonies/ng/109 cells)
|
|
|---|---|---|---|
| Avg | Range | ||
| gltB::ISlacZ/hah | E264 | 12 | 3.3-32 |
| BTH_I1592::ISlacZ/hah | E264 | 22 | 4.4-54 |
| Δ(gltB)::tet | E264 | 5.5 | 2.2-9.9 |
| Δ(mutS)::tet | E264 | 2.2 | 2.2 |
| Δ(mutS)::loxP-tmp-loxP | E264 | 20 | 13-26 |
| Δ(envZ-ompR)::tmp | E264 | 13 | 1.1-32 |
| Δ(amrRAB)::tmp | E264 | 20 | 5.5-38 |
| Δ(bpeEF-oprC)::tmp | E264 | 36 | 36 |
| Δ(gltB)::tet | S95019 | 190 | 76-330 |
| Δ(mutS)::tet | S95019 | 10 | 10 |
| Δ(mutS)::loxP-tmp-loxP | S95019 | 39 | 39b |
| Δ(envZ-ompR)::tmp | S95019 | 160 | 80-230 |
| Δ(amrRAB)::tmp | S95019 | 600 | 310-970 |
| Δ(bpeEF-oprC)::tmp | S95019 | 640 | 640 |
a
Chromosomal DNA was isolated from B. thailandensis strain E264 carrying the indicated mutations.
b
Three of three transformants were verified by PCR (Fig. 2).