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Accumulation of negative-stranded RNA of HcRNAV in Heterocapsa circularisquama strains HCLG-1 (A), HU9433-P (B), and MZ2 (C) inoculated with HcRNAV34 or HcRNAV109. Total RNA was extracted from H. circularisquama cells, separated by gel electrophoresis, and blotted onto membranes. The membranes were then probed with strand-specific digoxigenin-labeled RNA probes (22). Total RNA stained with SYBR gold (Molecular Probes, OR) is also shown. The assays were performed at least twice.
