FIG. 2.

Rating system for the onion pathogenesis assay used to type B. cepacia complex isolates. Onion slices were wounded, inoculated with 5 μl of bacterial culture (10⁷ CFU/ml), and incubated at 30°C for 48 h. A rating scale of 0 to 3 was used to indicate the degree of tissue maceration. A rating of 0 indicated no maceration, 1 indicated 1 to 33% macerated tissue area, 2 indicated 34% to 66% macerated tissue area, and 3 indicated 67% to 100% macerated tissue area.