Cellular fatty acid compositions of the mevalonate-producing strain (E. coli DP10 containing pBAD33MevT and pBAD18) and the inactive-pathway strain [E. coli DP10 containing pMevT(C159A) and pBAD18] obtained by FAME analysis. Open symbols in each graph represent the inactive-pathway strain, and solid symbols represent the mevalonate-producing strain. (A) Total fatty acids as a percentage of DCW. (B) UFA as a percentage of total fatty acids in each strain. ▴ and ▵, 16-carbon UFA (includes 16-carbon CFA); ▪ and □, 18-carbon UFA (includes 18-carbon CFA); and • and ○, CFA (both 16-carbon and 18-carbon CFA). (C) SFA as a percentage of total fatty acids. ▴ and ▵, 16-carbon SFA; ▪ and □, 14-carbon SFA; and • and ○, 12-carbon SFA.