Skip to main content
. 2008 Mar 14;74(10):3229–3241. doi: 10.1128/AEM.02750-07

FIG. 6.

FIG. 6.

Effect of fatty acid supplementation on growth of mevalonate-producing and control strains of E. coli DP10. (A) Growth of the active-pathway strain [E. coli DP10(pBAD33MevT)] in a defined medium supplemented with 100 μg/ml of oleic acid (cis-Δ9-18:1) (⋄), palmitoleic acid (cis-Δ9-16:1) (□), palmitic acid (16:0) (▵), a combination of fatty acids (16:0 plus cis-Δ9-16:1) (+), or no fatty acid supplement (○) in a 96-well incubator/plate reader monitored continuously at OD600. The growth of the inactive-pathway strain [E. coli DP10 carrying pMevT(C159A)] in nonsupplemented medium (▪) is shown as well. Growth profiles of the inactive-pathway control strain and an empty vector control [E. coli DP10(pBAD33)] were equivalent in medium supplemented with the various fatty acids. (B) Shake flask growth of E. coli DP10(pBAD33MevT) with (•) and without (○) a palmitic acid (16:0) supplement compared to that of the inactive-pathway control [E. coli DP10 carrying pMevT(C159A)] (▪).