TABLE 1.
Strains used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| DH10B | F−mcrA Δ(mrr-hsdRMS-mcrBC) F80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu)7697 galU galK λ−rpsL (Strr) nupG | Invitrogen |
| DP10 | DH10B Δ(araFGH) φ(ΔaraEp Pcp8-araE) | 51 |
| Plasmids | ||
| pBAD18 | Cloning vector containing Apr cassette, modified pBR322 origin with a truncated rop gene, araC, and PBAD | 22 |
| pBAD33 | Cloning vector containing Cmr cassette, pACYC184 origin, araC, and PBAD | 22 |
| pBAD33MevT | pBAD33 containing atoB, ERG13, and tHMGR under the control of PBAD; Cmr | 44, 51 |
| pMevT(C159A) | pBAD33MevT derivative containing ERG13(C159A) | 51 |