TABLE 1.

Oligonucleotide probes and hybridization conditions used in this study

Probea	Target group	Sequence (5′ to 3′)	Type	Formamide concn (%)	Hybridization temp/wash temp (°C)	Reference
ARCH915	Most Archaea	GTGCTCCCCCGCCAATTCCT	CARD	35	46/48	2
ANME-1-350	ANME-1	AGTTTTCGCGCCTGATGC	CARD	40	46/48	5
ANME-2-538	ANME-2	GGCTACCACTCGGGCCGC	FISH	50	46/48	82
ANME-3-1249	ANME-3	TCGGAGTAGGGACCCATT	CARD	20	46/48	41
EUB I	Most Bacteria	GCTGCCTCCCGTAGGAGT	CARD	35	46/48	2
EUB II	Planctomycetales	GCAGCCACCCGTAGGTGT	CARD	35	46/48	11
EUB III	Verrucomicrobiales	GCTGCCACCCGTAGGTGT	CARD	35	46/48	11
Non338	Negative hybridization probe	ACTCCTACGGGAGGCAGC	CARD-FISH	Variable	46/48	89
Alf968	Alphaproteobacteria	GGTAAGGTTCTGCGCGTT	FISH	35	46/48	55
Gam42	Gammaproteobacteria	GCCTTCCCACATCGTTT	FISH	35	46/48	46
Beta42a	Betaproteobacteria	GCCTTCCCACTTCGTTT	FISH	35	46/48	46
DSS658	Desulfosarcina/Desulfococcus	TCCACTTCCCTCTCCCAT	CARD	50	46/48	46
660	Desulfobulbus	GAATTCCACTTTCCCCTCTG	CARD	60	46/48	13
Mγ705	Type I methanotrophs	CTGGTGTTCCTTCAGATC	FISH	20	46/48	15
Arc94	Arcobacter	TGCGCCACTTAGCTGACA	CARD	20	46/48	73

^aEUB-I, -II, and -III were mixed to obtain a single solution designated EUB I-III.